Journal: PLoS ONE

Article Title: The Anti-Tumor Histone Deacetylase Inhibitor SAHA and the Natural Flavonoid Curcumin Exhibit Synergistic Neuroprotection against Amyloid-Beta Toxicity

doi: 10.1371/journal.pone.0085570

Figure Lengend Snippet: A. PC12 cells were treated with different concentrations of Aβ 25–35 (n = 5). *, p <0.05; ***, p <0.001 vs Ctrl. B. PC12 cells were treated with different concentrations of SAHA (n = 5). *, p <0.05; ***, p <0.001 vs Ctrl. C. PC12 cells were treated with different concentrations of curcumin (n = 5). *, p <0.05; ***, p <0.001 vs Ctrl. D. MTT assay was performed to detect cell viability after treating with SAHA and curcumin against Aβ 25–35 -induced cytotoxicity in PC12 cells (n = 5). ***, p <0.001 vs Ctrl; **, p <0.01 vs Aβ 25–35 .

Article Snippet: Rat pheochromocytoma PC12 cells were originally obtained from Chinapeptides Co., Ltd. PC12 neuron cells were cultured in DMEM-F12 containing 7% FBS, 1% penicillin, and 1% streptomycin at 37°C.

Techniques: MTT Assay

Journal: PLoS ONE

Article Title: The Anti-Tumor Histone Deacetylase Inhibitor SAHA and the Natural Flavonoid Curcumin Exhibit Synergistic Neuroprotection against Amyloid-Beta Toxicity

doi: 10.1371/journal.pone.0085570

Figure Lengend Snippet: A. Apoptotic cells were detected by TUNEL assay. Cells were stained with TUNEL positive nuclei (green) and nuclei of PC12 cells (blue). B. The percentage of TUNEL positive cells was determined (n = 5). ***, p <0.001 vs Ctrl; **, p <0.01 vs Aβ 25–35 . Ctrl: the control group. C. Western blot of cleaved caspase 3 (n = 3). ***, p <0.001 vs Ctrl; **, p <0.01 vs Aβ 25–35 . Ctrl: the control group; CASP3: caspase 3.

Article Snippet: Rat pheochromocytoma PC12 cells were originally obtained from Chinapeptides Co., Ltd. PC12 neuron cells were cultured in DMEM-F12 containing 7% FBS, 1% penicillin, and 1% streptomycin at 37°C.

Techniques: TUNEL Assay, Staining, Control, Western Blot

Journal: PLoS ONE

Article Title: The Anti-Tumor Histone Deacetylase Inhibitor SAHA and the Natural Flavonoid Curcumin Exhibit Synergistic Neuroprotection against Amyloid-Beta Toxicity

doi: 10.1371/journal.pone.0085570

Figure Lengend Snippet: A. Representative western blot of the p -Akt (Ser 473) protein expression with SAHA treatment (n = 3). **, p <0.01 vs Ctrl. B. Representative western blot of the p -Akt (Ser 473) protein expression with curcumin treatment (n = 3). **, p <0.01 vs Ctrl. C. Representative western blot of the p -Akt (Ser 473) protein expression with co-treatment of SAHA and curcumin (n = 3) to the PC12 cells (n = 3). *, p <0.05 vs Ctrl; **, p <0.01 vs Aβ 25–35 ; Ctrl: the control group.

Article Snippet: Rat pheochromocytoma PC12 cells were originally obtained from Chinapeptides Co., Ltd. PC12 neuron cells were cultured in DMEM-F12 containing 7% FBS, 1% penicillin, and 1% streptomycin at 37°C.

Techniques: Western Blot, Expressing, Control

Journal: Microorganisms

Article Title: Potential Effects of Indole-3-Lactic Acid, a Metabolite of Human Bifidobacteria, on NGF-Induced Neurite Outgrowth in PC12 Cells

doi: 10.3390/microorganisms8030398

Figure Lengend Snippet: Effects of indole-3-lactic acid (ILA) on nerve growth factor (NGF)-induced neurite outgrowth of PC12 cells. PC12 cells were treated with NGF (25 ng/mL) and the test compounds (RC, ILA or Trp at 1 nM, 10 nM, 100 nM, and 1 µM) for five consecutive days. ( A ) Percentage of neurite-bearing cells in PC12 cells. ( B ) Acetylcholinesterase (AchE) activity in PC12 cells. ( C ) Images of βIII-tubulin (green) immunostaining of PC12 cells treated with NGF (25 ng/mL) and the test compounds (RC, ILa or Trp at 100 nM) for five consecutive days at a magnification of ×100. Scale bars: 100 µm. Nuclei were counterstained with DAPI (blue). The data represent the mean ± SD of three replicates. One-way ANOVA with Tukey’s post hoc tests were used for statistical analysis. Means with different lowercase letters are significantly different between test groups ( p < 0.05). Means with different uppercase letters are significantly different within the same test group ( p < 0.05). NGF-treated control. NTC, nontreated control; papaverine hydrochloride was used as a reference control (RC); tryptophan (Trp) was used as a baseline compound.

Article Snippet: PC12 cell, a rat adrenal pheochromocytoma cell line, was purchased from the European Collection of Authenticated Cell Cultures (ECACC 88022401; Salisbury, UK).

Techniques: Activity Assay, Immunostaining

Journal: Microorganisms

Article Title: Potential Effects of Indole-3-Lactic Acid, a Metabolite of Human Bifidobacteria, on NGF-Induced Neurite Outgrowth in PC12 Cells

doi: 10.3390/microorganisms8030398

Figure Lengend Snippet: Effects of indole-3-lactic acid (ILA) on the phosphorylation of TrkA, ERK1/2, and CREB in PC12 cells. Phosphorylation of ( A ) TrkA, ( B ) ERK1/2, and ( C ) CREB in PC12 cells treated for 24 h with NGF (25 ng/mL) and RC, ILA, or Trp (100 nM) was detected using western blot analysis. The data represent the mean ± SD of three replicates. Means with different lowercase letters are significantly different between test groups ( p < 0.05) by one-way ANOVA with Tukey’s post hoc tests. NTC, nontreated control; Papavarine hydrochloride was used as a reference control (RC); Tryptophan (Trp) was used as a baseline compound.

Article Snippet: PC12 cell, a rat adrenal pheochromocytoma cell line, was purchased from the European Collection of Authenticated Cell Cultures (ECACC 88022401; Salisbury, UK).

Techniques: Western Blot

Journal: Microorganisms

Article Title: Potential Effects of Indole-3-Lactic Acid, a Metabolite of Human Bifidobacteria, on NGF-Induced Neurite Outgrowth in PC12 Cells

doi: 10.3390/microorganisms8030398

Figure Lengend Snippet: Effects of indole-3-lactic acid (ILA) on the aryl hydrocarbon receptor (AhR) in PC12 cells. PC12 cells were pretreated with the AhR antagonist, α-naphthoflavone (ANF; 1 µM), for 1 h and treated for five consecutive days with NGF (25 ng/mL) and RC, ILA or Trp (100 nM). Nonpretreated PC12 cells served as a null control. ( A ) AhR protein (95 kDa) in PC12 cells was detected by Western blot analysis using a monoclonal antibody specific for AhR. The corresponding β-actin blot served as a loading control. ( B ) Acetylcholinesterase (AchE) activity in PC12 cells. The data represent the mean ± SD of three replicates. * p < 0.05, ** p < 0.01 for intragroup differences compared by an independent Student’s t-test. Means with different lowercase letters are significantly different between test groups ( p < 0.05) by one-way ANOVA with Tukey’s post hoc tests. NTC, nontreated control; Papavarine hydrochloride was used as a reference control (RC); Tryptophan (Trp) was used as a baseline compound.

Article Snippet: PC12 cell, a rat adrenal pheochromocytoma cell line, was purchased from the European Collection of Authenticated Cell Cultures (ECACC 88022401; Salisbury, UK).

Techniques: Western Blot, Activity Assay

Journal: Microorganisms

Article Title: Potential Effects of Indole-3-Lactic Acid, a Metabolite of Human Bifidobacteria, on NGF-Induced Neurite Outgrowth in PC12 Cells

doi: 10.3390/microorganisms8030398

Figure Lengend Snippet: Effects of indole-3-lactic acid (ILA) on the aryl hydrocarbon receptor (AhR) in PC12 cells. PC12 cells were pretreated with the AhR antagonist, CH223191 (1 µM), for 1 h and treated for five consecutive days with NGF (25 ng/mL) and RC, ILA or Trp (100 nM). Nonpretreated PC12 cells served as a null control. ( A ) AhR protein (95 kDa) in PC12 cells was detected by Western blot analysis using a monoclonal antibody specific for AhR. The corresponding β-actin blot served as a loading control. ( B ) Acetylcholinesterase (AchE) activity in PC12 cells. The data represent the mean ± SD of three replicates. * p < 0.05, ** p < 0.01 for intragroup differences compared by an independent Student’s t -test. Means with different lowercase letters are significantly different between test groups ( p < 0.05) by one-way ANOVA with Tukey’s post hoc tests. NTC, nontreated control; Papavarine hydrochloride was used as a reference control (RC); Tryptophan (Trp) was used as a baseline compound.

Article Snippet: PC12 cell, a rat adrenal pheochromocytoma cell line, was purchased from the European Collection of Authenticated Cell Cultures (ECACC 88022401; Salisbury, UK).

Techniques: Western Blot, Activity Assay

Journal: Frontiers in Cell and Developmental Biology

Article Title: Detecting Key Functional Components Group and Speculating the Potential Mechanism of Xiao-Xu-Ming Decoction in Treating Stroke

doi: 10.3389/fcell.2022.753425

Figure Lengend Snippet: The comparation of effective components and non-KFCG components on cell viabilities induced PC12 cells. (A–E) The treatments with ferulic acid, vanillic acid, zingerone, caryophyllene oxide, and methylephedrine hydrochloride, respectively. (F) The structures of ferulic acid, vanillic acid, zingerone, caryophyllene oxide, methylephedrine hydrochloride, and edaravone. K represents the control group without the treatments of components and OGD; M represents the model group with OGD treatment and without components treatments; Eda represents edaravone (20 μM). ### represents the comparation of model group to control group ( p = 0.001); *, **, and *** represent the comparations of components groups to model group in the levels of p < 0.05, p < 0.01, and p < 0.001. (G) The screening process of components. Five components marked in red were selected for the in vitro experiments.

Article Snippet: The PC12 cell line was obtained from CHI SCIENTIFIC (Shanghai, China) and cultured in RPMI-1640 with FBS, penicillin 100 U/mL, streptomycin 100 μg/ml, respectively, at 37°C in a fully humidified 5% CO 2 atmosphere.

Techniques: Control, In Vitro